On a real-console, an old version of the TAS was ~17.9 seconds longer because of more lag, mostly concentrated in multi-character levels.
In other games by the same developer, out-of-bounds glitches are possible, but I couldn't find any setups in this game.The multi-character levels are particularly complex, so it was easy to lose time despite my best efforts.
However, this TAS is not perfect by any means.